Current chromosome conformation capture techniques do not offer sufficiently high resolution to interrogate these regulatory interactions on a genomic scale. *, Tavares-Cadete F.*, Young A. N.*, Sugar R., Schoenfelder S., Ferreira L., Wingett S. , Andrews S., Grey W., Ewels P.A., Herman B., Happe S., Higgs A., LeProust E., Follows G.A., Fraser P., Luscombe N.M., Osborne C.S.Nature Genetics; 2017 Mar;49(3):403-415 Warren H.R. *, Pazoki R.*, He G.*, Ntritsos G.*, Dimou N.* et alPLoS One; 2017 Apr 5;12(4):e0174744 Mifsud B. For example, promoter capture Hi-C is designed to enrich for interactions centered around a selected set of annotated promoters (Schoenfelder et al. Here we use Capture Hi-C (CHi-C), an adapted genome conformation assay, to examine the long-range interactions of almost 22,000 promoters in 2 human blood cell … Dr. Mifsud joined the William Harvey Research Insitute in QMUL as an MRC eMedLab Career Development Fellow and Lecturer in 2015, where she used chromatin interaction data to understand the function of regulatory GWAS mutations. In this context, more recently Bridge Linker-Hi-C (BL-Hi-C) was proposed as a solution to favor the detection of interactions mediated by structural or regulatory proteins. We developed Promoter Capture Hi-C (PCHi-C) to enable the genome-wide detection of distal promoter-interacting regions (PIRs), for all promoters in a single experiment.
Transcriptional control in large genomes often requires looping interactions between distal DNA elements, such as enhancers and target promoters. Here we use Capture Hi-C (CHi-C), an adapted genome conformation assay, to examine the long-range interactions of almost 22,000 promoters in 2 human blood cell types. Dr. Mifsud has been an Assistant Professor at HBKU since July 2018. Overview.
… *, et alGenome Research; 2015 Apr;25(4):582-97 Schoenfelder S.*, Furlan-Magaril M.*, Mifsud B. Nature Genetics; 2018 Sep; doi: 10.1038/s41588-018-0205-x Evangelou E.*, Warren H.R.
This study provides new insights and accessible tools to dissect the regulatory interactions that underlie normal and aberrant gene regulation. We identify over 1.6 million shared and cell type-restricted interactions spanning hundreds of kilobases between promoters and distal loci. We identify over 1.6 million shared and cell type-restricted interactions spanning hundreds of kilobases between promoters and distal loci. In her postdoctoral work she focussed on developing methods for the analysis of Hi-C type data and finding new biological insights into transcriptional regulation, by applying these methods to Hi-C and capture Hi-C data sets. 2015.
“Mapping Long-Range Promoter Contacts in Human Cells with High-Resolution Capture Hi-C.” Nature Genetics, May.
Here we use Capture Hi-C (CHi-C), an adapted genome conformation assay, to examine the long-range interactions of almost 22,000 promoters in 2 human blood cell types. and CHiCAGO called interactions from Mifsud et al. *, Evangelou E.*, Cabrera C.P.
Nature 506 (7488): 376–81. Transcriptionally active genes contact enhancer-like elements, whereas transcriptionally inactive genes interact with previously uncharacterized elements marked by repressive features that may act as long-range silencers.
*, Gao H.*, Ren M*., Mifsud B.